Seville is served by the TUSSAM (Transportes Urbanos de Sevilla) bus network which runs buses throughout the city as well as outlying areas surrounding Seville. El Metrocentro Tranvia is a tram line consisting of four stops, running from el Prado bus station, past the University and the Cathedral, and stopping at Plaza Nueva where the direction of service reverses.

On April 2, 2009, the city opened its first metro line, almost 28 months later than originally planned. The project experienced several delays caused by various reasons, including the relocation of archaeological findings and the need for a deeper tunnel under the Guadalquivir River, to avoid possible water leakages.

The Santa Justa train station is served by the AVE high-speed rail system, and is operated by the Spanish formerly state-owned rail company Renfe.

The Sevici community bicycle program has integrated bicycles into the public transport network. Across the city, bicycles are available for hire at low cost and green bicycle lanes can be seen on most major streets. This network of lanes is also currently being expanded.